The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. To select a dye that’s right for your application, see our Membrane and Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure. But unlike original CellBrite® dyes and lectins, CellBrite® Fix and MemBrite® Fix cannot be used on cells that are already fixed. MemBrite® Fix also can be used to stain yeast. MemBrite® Fix requires a two-step staining protocol with washing, but offers a more extensive choice of dye colors than CellBrite® Fix. MemBrite® Fix Cell Surface Stains do not bind lipids, but label cell surface proteins. CellBrite® Fix stains mammalian cells, yeast, and bacteria. Staining takes only 15 minutes in a single step with no wash. When they incorporate into lipids, they become fluorescent, and at the same time react covalently with membrane proteins for stable labeling. CellBrite® Fix Membrane Stains are fluorogenic reactive membrane dyes that rapidly accumulate at the plasma membrane. They are novel covalent stains that can be fixed and permeabilized for IF staining. CellBrite® NIR dyes are CellBrite® dyes with near-infrared fluorescence compatible with small animal NIR imaging systems.ĬellBrite® Fix and MemBrite® Fix stains were developed to overcome some of these shortcomings. The dyes also do not stain bacteria or yeast. But the staining has poor tolerance for permeabilization or methanol fixation, so CellBrite® staining is not easily combined with intracellular immunofluorescence (IF) staining. Cells can be fixed with formaldehyde before or after CellBrite® staining. CellBrite® Cytoplasmic Membrane Stains are lipophilic dyes for simple, non-toxic, stable labeling of membranes in live or fixed cells.
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